Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs freq...

Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability

Problems during DNA replication underlie genomic instability and drive malignant transformation. The DNA damage checkpoint stabilizes stalled replication forks thus counteracting aberrant fork transitions, DNA breaks and chromosomal rearrangements. We analyzed fork processing in checkpoint deficient cells by coupling psoralen crosslinking with replication intermediate two-dimensional gel analys...